Construct: shRNA TRCN0000217707

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175411.4-828s1c1
DNA Barcode:
GCAAGAGAAGGTCAGCTAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam118b (109229)
Gene Description:
family with sequence similarity 118, member B
Transcript:
RefSeq NM_175411.4 (NON-CURRENT)
Match location:
Position 828 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217707)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGAGAAGGTCAGCTAAAT
Hairpin Sequence:
5'-CCGG-GCAAGAGAAGGTCAGCTAAAT-CTCGAG-ATTTAGCTGACCTTCTCTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGAGAAGGTCAGCTAAATCTCGAGATTTAGCTGACCTTCTCTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGAGAAGGTCAGCTAAATCTCGAGATTTAGCTGACCTTCTCTTGC-3'

Other clones with same target sequence:

(none)