Construct: shRNA TRCN0000217718

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_619495.4-2462s1c1
DNA Barcode:
GTATCAGCTGAGTCATGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Thada (240174)
Gene Description:
thyroid adenoma associated
Transcript:
RefSeq XM_619495.4 (NON-CURRENT)
Match location:
Position 2462 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217718)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTATCAGCTGAGTCATGATAT
Hairpin Sequence:
5'-CCGG-GTATCAGCTGAGTCATGATAT-CTCGAG-ATATCATGACTCAGCTGATAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTATCAGCTGAGTCATGATATCTCGAGATATCATGACTCAGCTGATACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTATCAGCTGAGTCATGATATCTCGAGATATCATGACTCAGCTGATAC-3'

Other clones with same target sequence:

(none)