Construct: shRNA TRCN0000217755

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024193.2-548s1c1
DNA Barcode:
CCGAGTGGACAACATGATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nop56 (67134)
Gene Description:
NOP56 ribonucleoprotein
Transcript:
RefSeq NM_024193.2 (CURRENT)
Match location:
Position 548 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217755)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGTGGACAACATGATTAT
Hairpin Sequence:
5'-CCGG-CCGAGTGGACAACATGATTAT-CTCGAG-ATAATCATGTTGTCCACTCGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGTGGACAACATGATTATCTCGAGATAATCATGTTGTCCACTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGTGGACAACATGATTATCTCGAGATAATCATGTTGTCCACTCGG-3'

Other clones with same target sequence:

(none)