Construct: shRNA TRCN0000217786

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145511.2-566s1c1
DNA Barcode:
CCGAGAGCTAAGTTGGATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
BC003331 (226499)
Gene Description:
cDNA sequence BC003331
Transcript:
RefSeq NM_145511.2 (CURRENT)
Match location:
Position 566 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217786)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGAGCTAAGTTGGATAAT
Hairpin Sequence:
5'-CCGG-CCGAGAGCTAAGTTGGATAAT-CTCGAG-ATTATCCAACTTAGCTCTCGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGAGCTAAGTTGGATAATCTCGAGATTATCCAACTTAGCTCTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGAGCTAAGTTGGATAATCTCGAGATTATCCAACTTAGCTCTCGG-3'

Other clones with same target sequence:

(none)