Construct: shRNA TRCN0000217812

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026083.2-5373s1c1
DNA Barcode:
CGGCTAAGTCTTCGGTTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zc3h13 (67302)
Gene Description:
zinc finger CCCH type containing 13
Transcript:
RefSeq NM_026083.2 (CURRENT)
Match location:
Position 5373 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217812)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCTAAGTCTTCGGTTATTT
Hairpin Sequence:
5'-CCGG-CGGCTAAGTCTTCGGTTATTT-CTCGAG-AAATAACCGAAGACTTAGCCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCTAAGTCTTCGGTTATTTCTCGAGAAATAACCGAAGACTTAGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCTAAGTCTTCGGTTATTTCTCGAGAAATAACCGAAGACTTAGCCG-3'

Other clones with same target sequence:

(none)