Construct: shRNA TRCN0000217835

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023380.2-1493s1c1
DNA Barcode:
CATGGAGACCACGTACTAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Samsn1 (67742)
Gene Description:
SAM domain, SH3 domain and nuclear localization signals, 1
Transcript:
RefSeq NM_023380.2 (CURRENT)
Match location:
Position 1493 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217835)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGGAGACCACGTACTAATA
Hairpin Sequence:
5'-CCGG-CATGGAGACCACGTACTAATA-CTCGAG-TATTAGTACGTGGTCTCCATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGGAGACCACGTACTAATACTCGAGTATTAGTACGTGGTCTCCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGGAGACCACGTACTAATACTCGAGTATTAGTACGTGGTCTCCATG-3'

Other clones with same target sequence:

(none)