Construct: shRNA TRCN0000217905

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144552.3-933s1c1
DNA Barcode:
GCTTGCCATGAAGCATAAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stxbp6 (217517)
Gene Description:
syntaxin binding protein 6 (amisyn)
Transcript:
RefSeq NM_144552.3 (CURRENT)
Match location:
Position 933 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217905)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGCCATGAAGCATAAATG
Hairpin Sequence:
5'-CCGG-GCTTGCCATGAAGCATAAATG-CTCGAG-CATTTATGCTTCATGGCAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGCCATGAAGCATAAATGCTCGAGCATTTATGCTTCATGGCAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGCCATGAAGCATAAATGCTCGAGCATTTATGCTTCATGGCAAGC-3'

Other clones with same target sequence:

(none)