Construct: shRNA TRCN0000217945

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178890.3-3266s1c1
DNA Barcode:
GTGCTGTCAACACCTACAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Abtb2 (99382)
Gene Description:
ankyrin repeat and BTB (POZ) domain containing 2
Transcript:
RefSeq NM_178890.3 (CURRENT)
Match location:
Position 3266 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217945)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGCTGTCAACACCTACAAAT
Hairpin Sequence:
5'-CCGG-GTGCTGTCAACACCTACAAAT-CTCGAG-ATTTGTAGGTGTTGACAGCAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGCTGTCAACACCTACAAATCTCGAGATTTGTAGGTGTTGACAGCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGCTGTCAACACCTACAAATCTCGAGATTTGTAGGTGTTGACAGCAC-3'

Other clones with same target sequence:

  1. TRCN0000241924