Construct: shRNA TRCN0000218278

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016891.3-1690s21c1
DNA Barcode:
GATAGGACCCATTCTTGATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppp2r1a (51792)
Gene Description:
protein phosphatase 2, regulatory subunit A, alpha
Transcript:
RefSeq NM_016891.3 (CURRENT)
Match location:
Position 1690 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000218278)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATAGGACCCATTCTTGATAA
Hairpin Sequence:
5'-CCGG-GATAGGACCCATTCTTGATAA-CTCGAG-TTATCAAGAATGGGTCCTATC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATAGGACCCATTCTTGATAACTCGAGTTATCAAGAATGGGTCCTATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATAGGACCCATTCTTGATAACTCGAGTTATCAAGAATGGGTCCTATC-3'

Other clones with same target sequence:

(none)