Construct: shRNA TRCN0000218645

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005093.3-1415s21c1
DNA Barcode:
ATTACACCTTAGAGGATATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CBFA2T2 (9139)
Gene Description:
CBFA2/RUNX1 partner transcriptional co-repressor 2
Transcript:
RefSeq NM_005093.3 (CURRENT)
Match location:
Position 1415 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000218645)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTACACCTTAGAGGATATTG
Hairpin Sequence:
5'-CCGG-ATTACACCTTAGAGGATATTG-CTCGAG-CAATATCCTCTAAGGTGTAAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTACACCTTAGAGGATATTGCTCGAGCAATATCCTCTAAGGTGTAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTACACCTTAGAGGATATTGCTCGAGCAATATCCTCTAAGGTGTAAT-3'

Other clones with same target sequence:

(none)