Construct: shRNA TRCN0000219386

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013461.3-1172s1c1
DNA Barcode:
TGCCCTTCTCTGCCATCTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adra1a (11549)
Gene Description:
adrenergic receptor, alpha 1a
Transcript:
RefSeq NM_013461.3 (NON-CURRENT)
Match location:
Position 1172 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219386)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCCCTTCTCTGCCATCTTTG
Hairpin Sequence:
5'-CCGG-TGCCCTTCTCTGCCATCTTTG-CTCGAG-CAAAGATGGCAGAGAAGGGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCCCTTCTCTGCCATCTTTGCTCGAGCAAAGATGGCAGAGAAGGGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCCCTTCTCTGCCATCTTTGCTCGAGCAAAGATGGCAGAGAAGGGCA-3'

Other clones with same target sequence:

(none)