Construct: shRNA TRCN0000219402

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008174.2-848s1c1
DNA Barcode:
CCATCATGGTGGCTAACATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Grm8 (14823)
Gene Description:
glutamate receptor, metabotropic 8
Transcript:
RefSeq NM_008174.2 (CURRENT)
Match location:
Position 848 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219402)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCATGGTGGCTAACATTT
Hairpin Sequence:
5'-CCGG-CCATCATGGTGGCTAACATTT-CTCGAG-AAATGTTAGCCACCATGATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCATGGTGGCTAACATTTCTCGAGAAATGTTAGCCACCATGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCATGGTGGCTAACATTTCTCGAGAAATGTTAGCCACCATGATGG-3'

Other clones with same target sequence:

(none)