Construct: shRNA TRCN0000219409

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008174.2-2657s1c1
DNA Barcode:
CTTGTACTGTTTATGCCATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Grm8 (14823)
Gene Description:
glutamate receptor, metabotropic 8
Transcript:
RefSeq NM_008174.2 (CURRENT)
Match location:
Position 2657 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219409)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGTACTGTTTATGCCATTA
Hairpin Sequence:
5'-CCGG-CTTGTACTGTTTATGCCATTA-CTCGAG-TAATGGCATAAACAGTACAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGTACTGTTTATGCCATTACTCGAGTAATGGCATAAACAGTACAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGTACTGTTTATGCCATTACTCGAGTAATGGCATAAACAGTACAAG-3'

Other clones with same target sequence:

(none)