Construct: shRNA TRCN0000219433

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183031.2-695s1c1
DNA Barcode:
GCATGGAGTATCCAAACTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpr183 (321019)
Gene Description:
G protein-coupled receptor 183
Transcript:
RefSeq NM_183031.2 (CURRENT)
Match location:
Position 695 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219433)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGGAGTATCCAAACTTTG
Hairpin Sequence:
5'-CCGG-GCATGGAGTATCCAAACTTTG-CTCGAG-CAAAGTTTGGATACTCCATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGGAGTATCCAAACTTTGCTCGAGCAAAGTTTGGATACTCCATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGGAGTATCCAAACTTTGCTCGAGCAAAGTTTGGATACTCCATGC-3'

Other clones with same target sequence:

(none)