Construct: shRNA TRCN0000219519

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001481000.1-549s1c1
DNA Barcode:
CACGGGTGGACTCTTCAATAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tgif2-ps2 (634967)
Gene Description:
TGFB-induced factor homeobox 2, pseudogene 2
Transcript:
RefSeq XM_001481000.1 (NON-CURRENT)
Match location:
Position 549 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219519)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACGGGTGGACTCTTCAATAC
Hairpin Sequence:
5'-CCGG-CACGGGTGGACTCTTCAATAC-CTCGAG-GTATTGAAGAGTCCACCCGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACGGGTGGACTCTTCAATACCTCGAGGTATTGAAGAGTCCACCCGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACGGGTGGACTCTTCAATACCTCGAGGTATTGAAGAGTCCACCCGTG-3'

Other clones with same target sequence:

(none)