Construct: shRNA TRCN0000219583

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025816.2-1252s1c1
DNA Barcode:
TGCAGTGAATGTGCGAGATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tax1bp1 (52440)
Gene Description:
Tax1 (human T cell leukemia virus type I) binding protein 1
Transcript:
RefSeq NM_025816.2 (NON-CURRENT)
Match location:
Position 1252 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219583)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCAGTGAATGTGCGAGATAA
Hairpin Sequence:
5'-CCGG-TGCAGTGAATGTGCGAGATAA-CTCGAG-TTATCTCGCACATTCACTGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCAGTGAATGTGCGAGATAACTCGAGTTATCTCGCACATTCACTGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCAGTGAATGTGCGAGATAACTCGAGTTATCTCGCACATTCACTGCA-3'

Other clones with same target sequence:

(none)