Construct: shRNA TRCN0000219618

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025967.3-4238s1c1
DNA Barcode:
CCGCCTGATTCTCGCATTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
D16Ertd472e (67102)
Gene Description:
DNA segment, Chr 16, ERATO Doi 472, expressed
Transcript:
RefSeq NM_025967.3 (NON-CURRENT)
Match location:
Position 4238 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219618)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCCTGATTCTCGCATTAAT
Hairpin Sequence:
5'-CCGG-CCGCCTGATTCTCGCATTAAT-CTCGAG-ATTAATGCGAGAATCAGGCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCCTGATTCTCGCATTAATCTCGAGATTAATGCGAGAATCAGGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCCTGATTCTCGCATTAATCTCGAGATTAATGCGAGAATCAGGCGG-3'

Other clones with same target sequence:

(none)