Construct: shRNA TRCN0000219628

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023268.2-1353s1c1
DNA Barcode:
CGGTGCAGGCAAACCGATATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Qsox1 (104009)
Gene Description:
quiescin Q6 sulfhydryl oxidase 1
Transcript:
RefSeq NM_023268.2 (CURRENT)
Match location:
Position 1353 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219628)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTGCAGGCAAACCGATATA
Hairpin Sequence:
5'-CCGG-CGGTGCAGGCAAACCGATATA-CTCGAG-TATATCGGTTTGCCTGCACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTGCAGGCAAACCGATATACTCGAGTATATCGGTTTGCCTGCACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTGCAGGCAAACCGATATACTCGAGTATATCGGTTTGCCTGCACCG-3'

Other clones with same target sequence:

(none)