Construct: shRNA TRCN0000219629

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023268.2-2293s1c1
DNA Barcode:
TCTCTTACTTTGGTCTCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Qsox1 (104009)
Gene Description:
quiescin Q6 sulfhydryl oxidase 1
Transcript:
RefSeq NM_023268.2 (CURRENT)
Match location:
Position 2293 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219629)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTCTTACTTTGGTCTCTAAA
Hairpin Sequence:
5'-CCGG-TCTCTTACTTTGGTCTCTAAA-CTCGAG-TTTAGAGACCAAAGTAAGAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTCTTACTTTGGTCTCTAAACTCGAGTTTAGAGACCAAAGTAAGAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTCTTACTTTGGTCTCTAAACTCGAGTTTAGAGACCAAAGTAAGAGA-3'

Other clones with same target sequence:

(none)