Construct: shRNA TRCN0000219635

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178659.5-2165s1c1
DNA Barcode:
GGCTAGAGGCACAGTACTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Jmjd4 (194952)
Gene Description:
jumonji domain containing 4
Transcript:
RefSeq NM_178659.5 (NON-CURRENT)
Match location:
Position 2165 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219635)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCTAGAGGCACAGTACTAAA
Hairpin Sequence:
5'-CCGG-GGCTAGAGGCACAGTACTAAA-CTCGAG-TTTAGTACTGTGCCTCTAGCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCTAGAGGCACAGTACTAAACTCGAGTTTAGTACTGTGCCTCTAGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCTAGAGGCACAGTACTAAACTCGAGTTTAGTACTGTGCCTCTAGCC-3'

Other clones with same target sequence:

(none)