Construct: shRNA TRCN0000219642

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001220.3-244s1c1
DNA Barcode:
TACCAGCTCTACGAGGATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMK2B (816)
Gene Description:
calcium/calmodulin dependent protein kinase II beta
Transcript:
RefSeq NM_001220.3 (NON-CURRENT)
Match location:
Position 244 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219642)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACCAGCTCTACGAGGATATT
Hairpin Sequence:
5'-CCGG-TACCAGCTCTACGAGGATATT-CTCGAG-AATATCCTCGTAGAGCTGGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACCAGCTCTACGAGGATATTCTCGAGAATATCCTCGTAGAGCTGGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACCAGCTCTACGAGGATATTCTCGAGAATATCCTCGTAGAGCTGGTA-3'

Other clones with same target sequence:

(none)