Construct: shRNA TRCN0000219664

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001799.3-1015s1c2
DNA Barcode:
TAATCCATGTGCTCGAATTAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDK7 (1022)
Gene Description:
cyclin dependent kinase 7
Transcript:
RefSeq NM_001799.3 (NON-CURRENT)
Match location:
Position 1015 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219664)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAATCCATGTGCTCGAATTAC
Hairpin Sequence:
5'-CCGG-TAATCCATGTGCTCGAATTAC-CTCGAG-GTAATTCGAGCACATGGATTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAATCCATGTGCTCGAATTACCTCGAGGTAATTCGAGCACATGGATTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAATCCATGTGCTCGAATTACCTCGAGGTAATTCGAGCACATGGATTA-3'

Other clones with same target sequence:

  1. TRCN0000218391