Construct: shRNA TRCN0000219678

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002530.2-2641s1c1
DNA Barcode:
TGGTGGCTGGTGGTCATGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NTRK3 (4916)
Gene Description:
neurotrophic receptor tyrosine kinase 3
Transcript:
RefSeq NM_002530.2 (NON-CURRENT)
Match location:
Position 2641 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219678)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGTGGCTGGTGGTCATGAAT
Hairpin Sequence:
5'-CCGG-TGGTGGCTGGTGGTCATGAAT-CTCGAG-ATTCATGACCACCAGCCACCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGTGGCTGGTGGTCATGAATCTCGAGATTCATGACCACCAGCCACCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGTGGCTGGTGGTCATGAATCTCGAGATTCATGACCACCAGCCACCA-3'

Other clones with same target sequence:

(none)