Construct: shRNA TRCN0000219692

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002741.3-1992s1c1
DNA Barcode:
ACCTCTGACCCTCGAAGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PKN1 (5585)
Gene Description:
protein kinase N1
Transcript:
RefSeq NM_002741.3 (NON-CURRENT)
Match location:
Position 1992 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219692)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTCTGACCCTCGAAGATTT
Hairpin Sequence:
5'-CCGG-ACCTCTGACCCTCGAAGATTT-CTCGAG-AAATCTTCGAGGGTCAGAGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTCTGACCCTCGAAGATTTCTCGAGAAATCTTCGAGGGTCAGAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTCTGACCCTCGAAGATTTCTCGAGAAATCTTCGAGGGTCAGAGGT-3'

Other clones with same target sequence:

(none)