Construct: shRNA TRCN0000219714

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006609.3-997s1c1
DNA Barcode:
GTAGTGGAAGCAGTATCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP3K2 (10746)
Gene Description:
mitogen-activated protein kinase kinase kinase 2
Transcript:
RefSeq NM_006609.3 (NON-CURRENT)
Match location:
Position 997 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219714)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAGTGGAAGCAGTATCTTTA
Hairpin Sequence:
5'-CCGG-GTAGTGGAAGCAGTATCTTTA-CTCGAG-TAAAGATACTGCTTCCACTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAGTGGAAGCAGTATCTTTACTCGAGTAAAGATACTGCTTCCACTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAGTGGAAGCAGTATCTTTACTCGAGTAAAGATACTGCTTCCACTAC-3'

Other clones with same target sequence:

  1. TRCN0000332974