Construct: shRNA TRCN0000219767

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018013.3-644s1c1
DNA Barcode:
GCTATGATAAGGTTGAATTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SOBP (55084)
Gene Description:
sine oculis binding protein homolog
Transcript:
RefSeq NM_018013.3 (NON-CURRENT)
Match location:
Position 644 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219767)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATGATAAGGTTGAATTAA
Hairpin Sequence:
5'-CCGG-GCTATGATAAGGTTGAATTAA-CTCGAG-TTAATTCAACCTTATCATAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATGATAAGGTTGAATTAACTCGAGTTAATTCAACCTTATCATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATGATAAGGTTGAATTAACTCGAGTTAATTCAACCTTATCATAGC-3'

Other clones with same target sequence:

(none)