Construct: shRNA TRCN0000219787

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022445.3-642s1c1
DNA Barcode:
GTGATTGGTGTGGCCTTATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TPK1 (27010)
Gene Description:
thiamin pyrophosphokinase 1
Transcript:
RefSeq NM_022445.3 (NON-CURRENT)
Match location:
Position 642 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219787)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGATTGGTGTGGCCTTATTC
Hairpin Sequence:
5'-CCGG-GTGATTGGTGTGGCCTTATTC-CTCGAG-GAATAAGGCCACACCAATCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGATTGGTGTGGCCTTATTCCTCGAGGAATAAGGCCACACCAATCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGATTGGTGTGGCCTTATTCCTCGAGGAATAAGGCCACACCAATCAC-3'

Other clones with same target sequence:

(none)