Construct: shRNA TRCN0000219795

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198253.2-3572s1c1
DNA Barcode:
TGAGGCCTGAGTGAGTGTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TERT (7015)
Gene Description:
telomerase reverse transcriptase
Transcript:
RefSeq NM_198253.2 (NON-CURRENT)
Match location:
Position 3572 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219795)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAGGCCTGAGTGAGTGTTTG
Hairpin Sequence:
5'-CCGG-TGAGGCCTGAGTGAGTGTTTG-CTCGAG-CAAACACTCACTCAGGCCTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAGGCCTGAGTGAGTGTTTGCTCGAGCAAACACTCACTCAGGCCTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAGGCCTGAGTGAGTGTTTGCTCGAGCAAACACTCACTCAGGCCTCA-3'

Other clones with same target sequence:

(none)