Construct: shRNA TRCN0000219830

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001718217.1-1276s1c1
DNA Barcode:
TTCACGCCTTGCACGACATAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF534 (147658)
Gene Description:
zinc finger protein 534
Transcript:
RefSeq XM_001718217.1 (NON-CURRENT)
Match location:
Position 1276 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219830)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCACGCCTTGCACGACATAG
Hairpin Sequence:
5'-CCGG-TTCACGCCTTGCACGACATAG-CTCGAG-CTATGTCGTGCAAGGCGTGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCACGCCTTGCACGACATAGCTCGAGCTATGTCGTGCAAGGCGTGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCACGCCTTGCACGACATAGCTCGAGCTATGTCGTGCAAGGCGTGAA-3'

Other clones with same target sequence:

(none)