Construct: shRNA TRCN0000219840

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002650.2-2642s1c1
DNA Barcode:
ACGACATGATCCAGTACTATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PI4KA (5297)
Gene Description:
phosphatidylinositol 4-kinase alpha
Transcript:
RefSeq NM_002650.2 (NON-CURRENT)
Match location:
Position 2642 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219840)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGACATGATCCAGTACTATC
Hairpin Sequence:
5'-CCGG-ACGACATGATCCAGTACTATC-CTCGAG-GATAGTACTGGATCATGTCGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGACATGATCCAGTACTATCCTCGAGGATAGTACTGGATCATGTCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGACATGATCCAGTACTATCCTCGAGGATAGTACTGGATCATGTCGT-3'

Other clones with same target sequence:

  1. TRCN0000333095