Construct: shRNA TRCN0000219882

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032177.2-464s1c1
DNA Barcode:
CAATCCGAGACCTACAATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PHAX (51808)
Gene Description:
phosphorylated adaptor for RNA export
Transcript:
RefSeq NM_032177.2 (NON-CURRENT)
Match location:
Position 464 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219882)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAATCCGAGACCTACAATTAT
Hairpin Sequence:
5'-CCGG-CAATCCGAGACCTACAATTAT-CTCGAG-ATAATTGTAGGTCTCGGATTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAATCCGAGACCTACAATTATCTCGAGATAATTGTAGGTCTCGGATTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAATCCGAGACCTACAATTATCTCGAGATAATTGTAGGTCTCGGATTG-3'

Other clones with same target sequence:

(none)