Construct: shRNA TRCN0000219886

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002350.2-341s1c1
DNA Barcode:
AGACTCAACCAGTACGTAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LYN (4067)
Gene Description:
LYN proto-oncogene, Src family tyrosine kinase
Transcript:
RefSeq NM_002350.2 (NON-CURRENT)
Match location:
Position 341 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219886)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACTCAACCAGTACGTAATA
Hairpin Sequence:
5'-CCGG-AGACTCAACCAGTACGTAATA-CTCGAG-TATTACGTACTGGTTGAGTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACTCAACCAGTACGTAATACTCGAGTATTACGTACTGGTTGAGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACTCAACCAGTACGTAATACTCGAGTATTACGTACTGGTTGAGTCT-3'

Other clones with same target sequence:

  1. TRCN0000230902