Construct: shRNA TRCN0000219942

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006228.3-906s1c1
DNA Barcode:
CAGAACCTTCCCGTCTGATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PNOC (5368)
Gene Description:
prepronociceptin
Transcript:
RefSeq NM_006228.3 (NON-CURRENT)
Match location:
Position 906 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219942)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAACCTTCCCGTCTGATTG
Hairpin Sequence:
5'-CCGG-CAGAACCTTCCCGTCTGATTG-CTCGAG-CAATCAGACGGGAAGGTTCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAACCTTCCCGTCTGATTGCTCGAGCAATCAGACGGGAAGGTTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAACCTTCCCGTCTGATTGCTCGAGCAATCAGACGGGAAGGTTCTG-3'

Other clones with same target sequence:

(none)