Construct: shRNA TRCN0000219958

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032646.5-2419s1c1
DNA Barcode:
AGACACTTTGAAACGACTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TTYH2 (94015)
Gene Description:
tweety family member 2
Transcript:
RefSeq NM_032646.5 (NON-CURRENT)
Match location:
Position 2419 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219958)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACACTTTGAAACGACTTTA
Hairpin Sequence:
5'-CCGG-AGACACTTTGAAACGACTTTA-CTCGAG-TAAAGTCGTTTCAAAGTGTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACACTTTGAAACGACTTTACTCGAGTAAAGTCGTTTCAAAGTGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACACTTTGAAACGACTTTACTCGAGTAAAGTCGTTTCAAAGTGTCT-3'

Other clones with same target sequence:

(none)