Construct: shRNA TRCN0000219993

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017621.2-938s1c1
DNA Barcode:
GACTGATCCCGGGATTGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ALKBH4 (54784)
Gene Description:
alkB homolog 4, lysine demethylase
Transcript:
RefSeq NM_017621.2 (NON-CURRENT)
Match location:
Position 938 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219993)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTGATCCCGGGATTGAAAT
Hairpin Sequence:
5'-CCGG-GACTGATCCCGGGATTGAAAT-CTCGAG-ATTTCAATCCCGGGATCAGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTGATCCCGGGATTGAAATCTCGAGATTTCAATCCCGGGATCAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTGATCCCGGGATTGAAATCTCGAGATTTCAATCCCGGGATCAGTC-3'

Other clones with same target sequence:

(none)