Construct: shRNA TRCN0000220019

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006772.2-5571s1c1
DNA Barcode:
GGGCCAAATTTATACTTATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SYNGAP1 (8831)
Gene Description:
synaptic Ras GTPase activating protein 1
Transcript:
RefSeq NM_006772.2 (NON-CURRENT)
Match location:
Position 5571 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220019)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGCCAAATTTATACTTATAT
Hairpin Sequence:
5'-CCGG-GGGCCAAATTTATACTTATAT-CTCGAG-ATATAAGTATAAATTTGGCCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGCCAAATTTATACTTATATCTCGAGATATAAGTATAAATTTGGCCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGCCAAATTTATACTTATATCTCGAGATATAAGTATAAATTTGGCCC-3'

Other clones with same target sequence:

(none)