Construct: shRNA TRCN0000220093

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145687.x-1661s1c2, TRCN0000001831, NM_145687.x-1661s1c1
DNA Barcode:
CGAGAGGTGGAAGATAGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP4K4 (9448)
Gene Description:
mitogen-activated protein kinase kinase kinase kinase 4
Transcript:
RefSeq NM_145687.1 (NON-CURRENT)
Match location:
Position 1661 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220093)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGAGGTGGAAGATAGATTT
Hairpin Sequence:
5'-CCGG-CGAGAGGTGGAAGATAGATTT-CTCGAG-AAATCTATCTTCCACCTCTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGAGGTGGAAGATAGATTTCTCGAGAAATCTATCTTCCACCTCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGAGGTGGAAGATAGATTTCTCGAGAAATCTATCTTCCACCTCTCG-3'

Other clones with same target sequence:

(none)