Construct: shRNA TRCN0000220100

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177402.x-1029s1c2, TRCN0000001839, NM_177402.x-1029s1c1
DNA Barcode:
CGGGAAGCTCACTGTCTGCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SYT2 (127833)
Gene Description:
synaptotagmin 2
Transcript:
RefSeq NM_177402.3 (NON-CURRENT)
Match location:
Position 1029 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220100)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGAAGCTCACTGTCTGCAT
Hairpin Sequence:
5'-CCGG-CGGGAAGCTCACTGTCTGCAT-CTCGAG-ATGCAGACAGTGAGCTTCCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGAAGCTCACTGTCTGCATCTCGAGATGCAGACAGTGAGCTTCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGAAGCTCACTGTCTGCATCTCGAGATGCAGACAGTGAGCTTCCCG-3'

Other clones with same target sequence:

(none)