Construct: shRNA TRCN0000220111

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005704.x-4381s1c2, TRCN0000001850, NM_005704.x-4381s1c1
DNA Barcode:
CCGGAACTACAAACCCAACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTPRU (10076)
Gene Description:
protein tyrosine phosphatase receptor type U
Transcript:
RefSeq NM_005704.2 (NON-CURRENT)
Match location:
Position 4381 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220111)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGAACTACAAACCCAACAT
Hairpin Sequence:
5'-CCGG-CCGGAACTACAAACCCAACAT-CTCGAG-ATGTTGGGTTTGTAGTTCCGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGAACTACAAACCCAACATCTCGAGATGTTGGGTTTGTAGTTCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGAACTACAAACCCAACATCTCGAGATGTTGGGTTTGTAGTTCCGG-3'

Other clones with same target sequence:

(none)