Construct: shRNA TRCN0000220116

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005808.x-693s1c2, TRCN0000001855, NM_005808.x-693s1c1
DNA Barcode:
GCACAGTTCGTTTAAGCCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CTDSPL (10217)
Gene Description:
CTD small phosphatase like
Transcript:
RefSeq NM_005808.1 (NON-CURRENT)
Match location:
Position 693 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220116)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAGTTCGTTTAAGCCTAT
Hairpin Sequence:
5'-CCGG-GCACAGTTCGTTTAAGCCTAT-CTCGAG-ATAGGCTTAAACGAACTGTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAGTTCGTTTAAGCCTATCTCGAGATAGGCTTAAACGAACTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAGTTCGTTTAAGCCTATCTCGAGATAGGCTTAAACGAACTGTGC-3'

Other clones with same target sequence:

  1. TRCN0000314819