Construct: shRNA TRCN0000220132

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007026.x-407s1c2, TRCN0000001871, NM_007026.x-407s1c1
DNA Barcode:
TGCTACCATTGAGATCCCTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DUSP14 (11072)
Gene Description:
dual specificity phosphatase 14
Transcript:
RefSeq NM_007026.1 (NON-CURRENT)
Match location:
Position 407 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220132)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCTACCATTGAGATCCCTAA
Hairpin Sequence:
5'-CCGG-TGCTACCATTGAGATCCCTAA-CTCGAG-TTAGGGATCTCAATGGTAGCA-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCTACCATTGAGATCCCTAACTCGAGTTAGGGATCTCAATGGTAGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCTACCATTGAGATCCCTAACTCGAGTTAGGGATCTCAATGGTAGCA-3'

Other clones with same target sequence:

(none)