Construct: shRNA TRCN0000220144

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007079.x-373s1c2, TRCN0000001883, NM_007079.x-373s1c1
DNA Barcode:
CTACAAACACATGCGCTTCCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTP4A3 (11156)
Gene Description:
protein tyrosine phosphatase 4A3
Transcript:
RefSeq NM_007079.2 (NON-CURRENT)
Match location:
Position 373 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220144)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACAAACACATGCGCTTCCT
Hairpin Sequence:
5'-CCGG-CTACAAACACATGCGCTTCCT-CTCGAG-AGGAAGCGCATGTGTTTGTAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACAAACACATGCGCTTCCTCTCGAGAGGAAGCGCATGTGTTTGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACAAACACATGCGCTTCCTCTCGAGAGGAAGCGCATGTGTTTGTAG-3'

Other clones with same target sequence:

(none)