Construct: shRNA TRCN0000220157

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014011.x-1518s1c2, TRCN0000001896, NM_014011.x-1518s1c1
DNA Barcode:
GATCCCAGTTCGTGCATGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SOCS5 (9655)
Gene Description:
suppressor of cytokine signaling 5
Transcript:
RefSeq NM_014011.3 (NON-CURRENT)
Match location:
Position 1518 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220157)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCCCAGTTCGTGCATGTTT
Hairpin Sequence:
5'-CCGG-GATCCCAGTTCGTGCATGTTT-CTCGAG-AAACATGCACGAACTGGGATC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCCCAGTTCGTGCATGTTTCTCGAGAAACATGCACGAACTGGGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCCCAGTTCGTGCATGTTTCTCGAGAAACATGCACGAACTGGGATC-3'

Other clones with same target sequence:

(none)