Construct: shRNA TRCN0000220170

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_376917.1-718s1c2, TRCN0000020576, XM_376917.1-718s1c1
DNA Barcode:
CCTCTTCGTATCACTGGAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FUBP3 (8939)
Gene Description:
far upstream element binding protein 3
Transcript:
RefSeq XM_376917.1 (NON-CURRENT)
Match location:
Position 718 (CDS)
CRISPR Mechanism
CRISPR

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220170)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTTCGTATCACTGGAGAT
Hairpin Sequence:
5'-CCGG-CCTCTTCGTATCACTGGAGAT-CTCGAG-ATCTCCAGTGATACGAAGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTTCGTATCACTGGAGATCTCGAGATCTCCAGTGATACGAAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTTCGTATCACTGGAGATCTCGAGATCTCCAGTGATACGAAGAGG-3'

Other clones with same target sequence:

(none)