Construct: shRNA TRCN0000220192

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004492.1-253s1c2, TRCN0000020602, NM_004492.1-253s1c1
DNA Barcode:
CTTCAGTTTGATAAGGCTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GTF2A2 (2958)
Gene Description:
general transcription factor IIA subunit 2
Transcript:
RefSeq NM_004492.1 (NON-CURRENT)
Match location:
Position 253 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220192)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCAGTTTGATAAGGCTATA
Hairpin Sequence:
5'-CCGG-CTTCAGTTTGATAAGGCTATA-CTCGAG-TATAGCCTTATCAAACTGAAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCAGTTTGATAAGGCTATACTCGAGTATAGCCTTATCAAACTGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCAGTTTGATAAGGCTATACTCGAGTATAGCCTTATCAAACTGAAG-3'

Other clones with same target sequence:

  1. TRCN0000297812