Construct: shRNA TRCN0000220193

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004492.1-449s1c2, TRCN0000020603, NM_004492.1-449s1c1
DNA Barcode:
CTGGCTCCAATACTACAGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GTF2A2 (2958)
Gene Description:
general transcription factor IIA subunit 2
Transcript:
RefSeq NM_004492.1 (NON-CURRENT)
Match location:
Position 449 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220193)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGCTCCAATACTACAGAAT
Hairpin Sequence:
5'-CCGG-CTGGCTCCAATACTACAGAAT-CTCGAG-ATTCTGTAGTATTGGAGCCAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGCTCCAATACTACAGAATCTCGAGATTCTGTAGTATTGGAGCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGCTCCAATACTACAGAATCTCGAGATTCTGTAGTATTGGAGCCAG-3'

Other clones with same target sequence:

  1. TRCN0000280279