Construct: shRNA TRCN0000220226

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138457.1-1919s1c2, TRCN0000020636, NM_138457.1-1919s1c1
DNA Barcode:
GTTCGCCTATTTCCGCAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FOXP4 (116113)
Gene Description:
forkhead box P4
Transcript:
RefSeq NM_138457.1 (NON-CURRENT)
Match location:
Position 1919 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220226)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCGCCTATTTCCGCAGAAA
Hairpin Sequence:
5'-CCGG-GTTCGCCTATTTCCGCAGAAA-CTCGAG-TTTCTGCGGAAATAGGCGAAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCGCCTATTTCCGCAGAAACTCGAGTTTCTGCGGAAATAGGCGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCGCCTATTTCCGCAGAAACTCGAGTTTCTGCGGAAATAGGCGAAC-3'

Other clones with same target sequence:

(none)