Construct: shRNA TRCN0000220234

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006902.2-1355s1c2, TRCN0000020646, NM_006902.2-1355s1c1
DNA Barcode:
GCAGGCTTTGGAGCGTGTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRRX1 (5396)
Gene Description:
paired related homeobox 1
Transcript:
RefSeq NM_006902.2 (NON-CURRENT)
Match location:
Position 1355 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220234)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGCTTTGGAGCGTGTCTT
Hairpin Sequence:
5'-CCGG-GCAGGCTTTGGAGCGTGTCTT-CTCGAG-AAGACACGCTCCAAAGCCTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGCTTTGGAGCGTGTCTTCTCGAGAAGACACGCTCCAAAGCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGCTTTGGAGCGTGTCTTCTCGAGAAGACACGCTCCAAAGCCTGC-3'

Other clones with same target sequence:

(none)