Construct: shRNA TRCN0000220286

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009218.1-1012s1c2, TRCN0000026848, NM_009218.1-1012s1c1
DNA Barcode:
CTAAGACCATCACGTCGCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sstr3 (20607)
Gene Description:
somatostatin receptor 3
Transcript:
RefSeq NM_009218.1 (NON-CURRENT)
Match location:
Position 1012 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220286)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAAGACCATCACGTCGCATT
Hairpin Sequence:
5'-CCGG-CTAAGACCATCACGTCGCATT-CTCGAG-AATGCGACGTGATGGTCTTAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAAGACCATCACGTCGCATTCTCGAGAATGCGACGTGATGGTCTTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAAGACCATCACGTCGCATTCTCGAGAATGCGACGTGATGGTCTTAG-3'

Other clones with same target sequence:

(none)