Construct: shRNA TRCN0000220291

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181753.2-690s1c2, TRCN0000026841, NM_181753.2-690s1c1
DNA Barcode:
TCTTCTAAAGAGGTAGCCCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Opn5 (353344)
Gene Description:
opsin 5
Transcript:
RefSeq NM_181753.2 (NON-CURRENT)
Match location:
Position 690 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220291)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTTCTAAAGAGGTAGCCCAT
Hairpin Sequence:
5'-CCGG-TCTTCTAAAGAGGTAGCCCAT-CTCGAG-ATGGGCTACCTCTTTAGAAGA-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTTCTAAAGAGGTAGCCCATCTCGAGATGGGCTACCTCTTTAGAAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTTCTAAAGAGGTAGCCCATCTCGAGATGGGCTACCTCTTTAGAAGA-3'

Other clones with same target sequence:

(none)